| Autor: |
Petersen, W. L., Sulc, S., Armstrong, C. L. |
| Zdroj: |
Plant Cell Reports; January 1992, Vol. 10 Issue: 12 p591-594, 4p |
| Abstrakt: |
Fertile plants have been obtained from maize (Zea mays L.) embryogenic suspension culture protoplasts. Friable, embryogenic callus initiated from an immature embryo from a cross involving the genotypes A188, B73, and Black Mexican sweetcorn was used to establish a rapidly growing embryogenic suspension culture. After nine months in culture, high yields of viable protoplasts (30×106/ gram fresh weight) were obtained following a 1.5 hour enzymatic digestion. Protoplasts cultured with feeder cells divided and formed embryogenic callus, from which male and female fertile plants were regenerated. Protoplast-derived R1 plants were self-pollinated and immature R2 embryos isolated for callus initiation. Female fertile plants have also been produced from protoplasts isolated from an R2-derived suspension culture. Significant interactions between protoplast and feeder-cell lines were observed. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
Full text from SpringerLink