Genetic transformation of grapevine cells

Autor: Baribault, T. J., Skene, K. G. M., Steele Scott, N.
Zdroj: Plant Cell Reports; March 1989, Vol. 8 Issue: 3 p137-140, 4p
Abstrakt: Biovar 1 strains ofAgrobacterium tumefaciens have been used to transform a cell suspension culture ofVitis vinifera cv. Cabernet Sauvignon. Cocultivation of cultures withAgrobacterium strains bearing either the cointegrate pGV3850::1103neo, or the binary vector pGA474-68, each gave rise to kanamycin resistant tissue. The stable integration and expression of the neomycin phosphotransferase gene was confirmed by Southern blotting and enzymic assay, respectively.
Databáze: Supplemental Index