| Autor: |
Chand, P. K., Rech, E. L., Golds, T. J., Power, J. B., Davey, M. R. |
| Zdroj: |
Plant Cell Reports; February 1989, Vol. 8 Issue: 2 p86-89, 4p |
| Abstrakt: |
Freshly isolated cell suspension protoplasts ofSolanum dulcamara were mixed withAgrobacterium rhizogenes, allowed to settle for 2 h, exposed to electrical pulses and further incubated for 2h. Two pulses of 600 V cm-1 for 2 msec separated by 15 sec produced transformed colonies at relative and absolute transformation frequencies which were 3–4 and 10 fold greater than those obtained by co-cultivation of 3 days old protoplast-derived cells with bacteria. Transformed colonies were not produced when freshly isolated protoplasts were mixed withAgrobacterium but not electroporated. Biochemical analysis confirmed the transgenic nature of plants regenerated from protoplast-derived tissues. |
| Databáze: |
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