Bialaphos resistance as a dominant selectable marker in Neurospora crassa

Autor: Avalos, Javier, Geever, Robert F., Case, Mary E.
Zdroj: Current Genetics; December 1989, Vol. 16 Issue: 5-6 p369-372, 4p
Abstrakt: Conidia of Neurospora crassa are sensitive to the herbicide bialaphos at concentrations of 160 mg/1 in Westergaard's or Fries' minimal media. Plasmid pJA4 was constructed by inserting a truncated bar gene from Streptomyces hygroscopicus fused to the his-3 promoter from N. crassa into pUC19. The bar gene in plasmid pJA4 confers resistance to bialaphos when transformants are selected on a medium containing bialaphos. The bar gene can be used as an additional dominant selectable marker for transformation of fungi.
Databáze: Supplemental Index