| Autor: |
Arshavskii, Yu. I., Gel'fand, I. M., Delyagina, T. G., Orlovskii, G. N., Pavlova, G. A., Panchin, Yu. V., Popova, L. B. |
| Zdroj: |
Neurophysiology; January 1987, Vol. 19 Issue: 1 p70-75, 6p |
| Abstrakt: |
Neurons from the peripharyngeal nerve ring detached from the pteropodial molluskClione limacina were cultured in polylysine prepared in a 25% Leibovitz saltwater medium containing 2% embryonic calf serum. Neurite outgrowth was observed in a proportion of the neurons, reaching its peak rate during the first three days. Neurites grew up to 300 µm in length. Membrane potential of the neurons measured 40–60 mV. The latter generated either single action potentials or volleys of spikes. Neural connections between 70 pairs of cells with overlapping neurites were investigated on days 3–4. An electrical connection was discovered between cells in 20% of the pairs examined and, in 6%, stimulating one neuron of a pair produced an inhibitory postsynaptic potential in the other. |
| Databáze: |
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