Autor: |
Hayashi, Kazuhiko, Ohara, Nobuya, Fujiwara, Kotaro, Jeon, Ho Jong, Koirala, Tirtha Raj, Takahashi, Kiyoshi, Akagi, Tadaatsu, Tomita, Noriko, Miyamoto, Kanji |
Zdroj: |
Virchows Archiv B Cell Pathology Zell-pathologie; December 1993, Vol. 63 Issue: 1 p37-41, 5p |
Abstrakt: |
In order to discriminate HTLV-II from HTLV-I, HTLV-II-specific polyclonal antibodies against a synthetic peptide of HTLV-II envelope sequence were raised in rabbits. We immunized two adult rabbits with a KLH-conjugated synthetic peptide corresponding to the amino acid sequence 171–196 of the HTLV-II envelope sequence, which is a specific region for HTLV-II as evaluated with an ELISA method. The resulting rabbit antisera to the synthetic peptide reacted with gp46 of HTLV-II lysates in Western blot analysis, but not with that of HTLV-I. Flow cytometric analysis and immunohistochemical study revealed that these affinity purified antisera recognized some HTLV-II-producing cell lines examined, but not HTLV-I-producing cell lines or other cell lines uninfected by HTLV. These findings indicate that these antisera specifically recognized the envelope glycoprotein (gp46) of HTLV-II and suggest the specificity of this region in the immune response to HTLV-II. Such antisera are useful in distinguishing between HTLV-I and HTLV-II infection and in determining the presence of individual HTLV-II-infected cells both in vivo and in vitro, including non-lymphoid cells. They may also assist in the elucidation of the pathogenesis of HTLV-II. |
Databáze: |
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