| Abstrakt: |
Immature leaf tissue of two commercial clones of sugarcane was subjected to tissue culture in Louisiana and Hawaii. The callus-incuding activity of selected compounds at 2, 4, and 20 mg/l was compared to the activity of 2,4-D at the same concentrations and to tissue on medium without a callus-inducing agent. Of the 79 compounds tested, 25 induced callus and 54 were ineffective. Ninety-six percent of the effective compounds were in chemical groups with known auxin activity. The results suggested that simple ring structure, phenol derivatives, or a halogenated hydrocarbon chain are not sufficient to induce callus. When callus was transferred to a medium lacking the inductive chemical, differentiation into shoots, roots, or both occurred in callus produced by 80% of the effective compounds. |
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