| Autor: |
Liou, G. I., Fong, S. -L., Gosden, J., van Tuinen, P., Ledbetter, D. H., Christie, S., Rout, D., Bhattacharya, S., Cook, R. G., Li, Y., Wang, C., Bridges, C. D. B. |
| Zdroj: |
Somatic Cell and Molecular Genetics; July 1987, Vol. 13 Issue: 4 p315-323, 9p |
| Abstrakt: |
A cloned 2184-bp cDNA coding for human interstitial retinol-binding protein (IRBP) has been isolated and sequenced. The probe hybridized to a 5.2-kb poly(A) RNA from human retinas. Nineteen tryptic peptides (363 amino acids) sequenced and purified from bovine IRBP could be aligned with 86–88% homology to the translated sequence. Two segments approximately 200 amino acids long were found to have a 41% residue identity,suggesting an internal duplication event. This cloned cDNA was used to probe DNA samples from a panel of 29 rodent-human somatic cell hybrids, mapping the structural gene for IRBP to chromosome 10. In situ hybridization suggested a regional localization near the centromere (p11.2?q11.2), although a secondary site of hybridization at q24?25 was also observed. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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