| Autor: |
Durnam, Diane M., Gelinas, Richard E., Myerson, David |
| Zdroj: |
Somatic Cell and Molecular Genetics; November 1985, Vol. 11 Issue: 6 p571-577, 7p |
| Abstrakt: |
We describe an in situ hybridization technique which allows rapid identification of species-specific chromosomes in somatic cell hybrid lines. Chromosome preparations from rodent-human hybrid lines are hybridized to biotinylated total human DNA which is subsequently detected by a series of immunocytochemical reactions which culminate in a peroxidase reaction visible by light microscopy. This technique not only allows identification of intact human chromosomes but also fragmented and rearranged human chromosomal segments. We have detected as little as 1 x 107 bp of human DNA inserted into a mouse chromosome using this procedure and estimate that the sensitivity of the technique would allow detection of sequences 5- to 10-fold smaller. The usefulness of the technique for screening hybrid cell gene mapping panels is discussed. |
| Databáze: |
Supplemental Index |
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