| Autor: |
Hsie, Abraham W., Brimer, Patricia A., Mitchell, Toby J., Gosslee, David G. |
| Zdroj: |
Somatic Cell and Molecular Genetics; October 1975, Vol. 1 Issue: 4 p383-389, 7p |
| Abstrakt: |
Exposure of Chinese hamster ovary (CHO) cells clone K1BE4 to ultraviolet (UV) light at doses up to 86 ergs/mm2 did not significantly reduce cell survival, but UV doses of 86–648 ergs/mm2 produced an exponential cell killing. Observed mutation frequency to 6-thioguannine resistance induced by UV increases approximately in proportion to increasing doses up to 260 ergs/mm2 in a range of 5–648 ergs/mm2 examined. The pooled data of mutation frequency f(X) as a function of dose X from 0–260 ergs/mm2 is adequately described by f(X)=10-6 (13.6+2.04 X). That the UVinduced mutations to 6-thioguanine resistance affects the hypoxanthineguanine phosphoribosyltransferase (HGPRT) locus is supported by the observation that all randomly isolated drugresistant colonies contained highly reduced or undetectable HGPRT activity. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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