| Autor: |
Fisch, Benjamin, Kraicer, Ruth Kaplan, Amit, Shoshana, Zukerman, Zvi, Ovadia, Jardena, Tadir, Yona |
| Zdroj: |
Journal of Assisted Reproduction and Genetics; February 1990, Vol. 7 Issue: 1 p38-44, 7p |
| Abstrakt: |
The relationship between sperm parameters and fertilizing capacity in vitro was examined retrospectively, with the aim of finding predictive criteria for successful in vitro fertilization. Three hundred thirty semen samples were used to inseminate 1462 oocytes. Conventional parameters of sperm concentration and percentage motility in the ejaculate as well as swim-up migration were analyzed in relation to fertilization rate. It was shown that the probability of fertilizing human oocytes in vitro decreases significantly when (a) the sperm concentration is below 20×106 spermatozoa/ml ejaculate (P=0.006), (b) motility is lower than 80% (P=0.002), or (c) less than 4×106 motile spermatozoa/ml are concentrated in the swim-up fraction (P<0.0001). It was also demonstrated that nonfertilizing sperm could not be distinguished from fertilizing sperm by the conventional criteria but rather by the average concentration of motile spermatozoa in the swim-up fraction [12.5±1.5 and 22.3±2.3×106/ml for the 0 and the 100% fertilization groups, respectively (mean±SE;P<0.01)]. Thus, the swim-up migration technique can serve as a predictive test for the in vitro fertilizing capacity of sperm. |
| Databáze: |
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