| Abstrakt: |
Anti-Pr cold agglutinins (CAs) with the subspecificities anti-Pr1h,-Pr1d, -Pr2, -Pr3h, -Pr3d, -PrMand anti-Sa CAs recognize immunodominantN-acetylneuraminic acid (NeuNAc) groups of tetra and/or trisaccharides (O-glycans) of glycophorin. These O-glycans are sialylated in α2,3- and/or α2,6-linkages. Sa and most Pr antigens have been inactivated by α2,3-specific sialidases. Antigenicity was reconstituted on desialylated glycophorin by α2,3-specific Galβ1,3GalNAc-sialyltransferase indicating that α2,3-linked NeuNAc groups are the immunodominant components of Sa and most Pr antigens. Some Pr antigens were resistant to α2,3-specific sialidase and were not reconstituted by α2,3-specific Galβ1,3GalNAc-sialyltransferase, which indicates that α2,6-linked NeuNAc group represents an immunodominant component of some Pr antigens. |
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