Monoclonal antibodies specific for type 3 and type 4 chain-based blood group determinants: Relationship to the A1 and A2 subgroups

Autor: Pendu, Jacques, Lambert, Francine, Samuelsson, Bo, Breimer, Michael, Christiane Seitz, R, Urdaniz, Maria, Suesa, Nuria, Ratcliffe, Murray, Francois, Anne, Poschmann, Alois, Vinas, Josep, Oriol, Rafael
Zdroj: Glycoconjugate Journal; September 1986, Vol. 3 Issue: 3 p255-271, 17p
Abstrakt: Two monoclonal antibodies, specific for A type 3 and A type 4 blood group determinants, are described. These antibodies recognized A1 but not A2 erythrocytes. A third monoclonal antibody showing specificity for A type 3 and A type 4, and also for H type 3 and H type 4, did not discriminate between A1 and A2 erythrocytes. On red cells these three antibodies recognized glycosphingolipids and binding to glycoproteins could not be demonstrated. On paraffin-embedded tissue sections the three antibodies labelled a supranuclear area, characteristic of the Golgi apparatus, of all cells producing A antigens. This labelling occurred irrespective of the A1, A2 status. The results suggest that glycolipids of erythrocytes and possibly of other cell types bear the A type 3/4 determinant specific for the A1 subgroup and that A type 3/4 determinants of glycoproteins might be present in both A1 and A2 subgroups on short oligosaccharide chains which are only detectable at the level of the Golgi apparatus.
Databáze: Supplemental Index
Externí odkaz: