The effects of carbodiimides on functions associated with the energy-conservation mechanism in beef heart sub-mitochondrial particles

Autor: Beechey, R. B., Knight, I. G.
Zdroj: Journal of Bioenergetics and Biomembranes; August 1978, Vol. 10 Issue: 3-4 p89-100, 12p
Abstrakt: N,N'-di-n-propyl-,N,N'di-n-butyl-,N,N'-di-n-pentyl-,N,N'di-n-hexyl-,N,N'di-n-octoyl-,N,N'-dibenzhydryl-, andN,N'-dibenzhydrylcarbodiimides were synthesized. They were all effective inhibitors (2 nmoles carbodiimide per milligram protein) of the ATP-driven reduction of NAD by succinate and the ATP-driven transhydrogenase activities catalyzed by beef heart submitochondrial particles (SMP). They had no effect on the nonenergylinked transhydrogenase and stimulated the succinate-driven aerobic transhydrogenase activity of beef heart SMP. It was concluded that they exert their effects by reacting with theN,N'-dicyclohexylcarbodiimide-binding protein. Water-soluble carbodiimides were not effective inhibitors.
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