| Autor: |
Markovič, Oskar, Stovíčková, Jana, Jörnvall, Hans |
| Zdroj: |
Journal of Protein Chemistry; February 1996, Vol. 15 Issue: 2 p127-130, 4p |
| Abstrakt: |
The role of histidine residues in pectinesterases was evaluated by monitoring the sensitivity to modification with diethyl pyrocarbonate in the tomato andAspergillus nigerenzymes. Different and incomplete losses of enzyme activity were obtained. Inactivation of the enzymes was proportional to the histidine content (two in the tomato T1 form, six in theAspergillusform), suggesting that accessible histidine residues do not have active-site functions in these pectinesterases, but contribute to the overall structural stability. Lack of His roles in common between the enzyme forms is in agreement with the structures of pectinesterases having no conserved His residues. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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