| Autor: |
Samiwala, E., Joshi, Vasudha, Notani, N. |
| Zdroj: |
Journal of Biosciences; September 1988, Vol. 13 Issue: 3 p223-228, 6p |
| Abstrakt: |
Using DNA clones, the physical distance between the linked genesnovandstrinHaemophilus influenzaewas estimated. Although none of the cloned inserts contained both the markers, pJ1-8StrR13 (insert of 18·7 kb) includedstrgene at one end and part ofnovgene at the other end of the insert. By EcoRI restriction analysis and by Southern hybridization, the distance between the two EcoRI sites, cutting at which inactivates the two genes, was estimated to be 17·7 kb. A single continuous EcoRI fragment (containing 4EcoRI sites within it) carrying both the genes intact would need to be 20·4 kb in size. These estimates were confirmed independently using different clones ofnovrandstrralleles as probes for hybridization with BamHI-digested chromosomal DNA. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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