| Autor: |
Tada, Mitsuhiro, Suzuki, Kazuo, Yamakawa, Yoshio, Sawamura, Yutaka, Sakuma, Shirou, Abe, Hiroshi, van Meir, Erwin, de Tribolet, Nicolas |
| Zdroj: |
Journal of Neuro-Oncology; April 1993, Vol. 16 Issue: 1 p25-34, 10p |
| Abstrakt: |
Summary The production of interleukin 8 (IL-8), a neutrophil chemotactic factor, and its amino acid sequence were examined in glioblastoma cell linesin vitro. Neutrophil chemotactic activity was demonstrated in 9 conditioned media of 15 human glioblastoma cell lines. Tumor necrosis factor (TNF)-a stimulated secretion of the activity in 7 lines and induced secretion in 4 other lines. ELISA quantification disclosed that the conditioned media contained interleukin 8 (IL-8) in an amount equivalent to the chemotactic activity. The IL-8 secretion increased with the stimulation by TNF-a. Northern blot analysis and the RT-PCR method confirmed expression of mRNA in the glioblastoma cells and its augmentation by TNF-a and/or IL-ß. Reversed-phase HPLC following ion-exchange chromatography revealed that the chemotactic activity was a single peptide, which was determined to be IL-8 by the retention time and ELISA. Furthermore, amino acid analysis disclosed that a major part of the glioblastoma-cell derived IL-8 peptide was 77 amino acid IL-8 (IL-877; with the N-terminal sequence AVLPRSAKELRCQCI-). |
| Databáze: |
Supplemental Index |
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