Cross-linking of protein by ω-maleimido alkanoylN-hydroxysuccinimido esters

Autor: Partis, M. D., Griffiths, D. G., Roberts, G. C., Beechey, R. B.
Zdroj: Journal of Protein Chemistry; June 1983, Vol. 2 Issue: 3 p263-277, 15p
Abstrakt: A series of chemical bifunctional cross-linking reagents, the succinimidyl maleimides, has been synthesized. Using hemoglobin as a model protein, it has been shown that these molecules react rapidly with sulfhydryl groups and more slowly with amino groups. The result is a high degree of specific cross-linking between the intramolecular subunits.
Databáze: Supplemental Index