| Autor: |
Escobar, Aldo, Gaete, Victoria, Núñez, Marco Tulio |
| Zdroj: |
Journal of Bioenergetics and Biomembranes; April 1992, Vol. 24 Issue: 2 p227-233, 7p |
| Abstrakt: |
Externally added ascorbate or NADH effectively reduced ferricyanide and promoted the exit of Fe3+ originated from acid-destabilized transferrin contained inside endocytic vesicles. The effect of ascorbate was mediated by an ascorbate uptake system, and the effect of NADH was mediated by the membrane-associated oxidoreductase. At physiological concentrations of both ascorbate and NADH, the ascorbate transport and the NADH-oxidoreductase system were additive as measured by the rate of reduction of ferricyanide and by the mobilization of transferrin-associated iron. The results indicate that Fe3+ reduction may occur by a nonenzymatic reaction with ascorbate transported into the vesicle lumen. The ascorbate-mediated reduction of iron derived from transferrin occurring in the endosome could play a major role in cellular iron uptake. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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