Histone H10 mRNA and protein accumulate early during retinoic acid induced differentiation of synchronized embryonal carcinoma cells

Autor: Hemert, F. J., Jonk, L. J. C., Destrée, O. H. J.
Zdroj: Molecular Biology Reports; February 1992, Vol. 16 Issue: 1 p33-38, 6p
Abstrakt: The very lysine-rich replacement histone variant H10 is found to be present in different murine (C1003, PC13, P19) and human (Tera-2) embryonal carcinoma cell lines. The proportion of H10 increases upon induction of differentiation of the different cell lines by various treatments. In undifferentiated PC13 EC cells H10 mRNA is present at a low level. During retinoic acid induced differentiation of mitotically synchronized PC13 EC cells, accumulation of H10 mRNA starts in the first cell cycle. The H10 protein level starts to increase in the second synchronous cycle preceding changes in the cycle parameters that become apparent in the third cycle. The results provide further support for an important role of H10 in the control of cellular differentiation in early mammalian development.
Databáze: Supplemental Index
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