Specificity of factors isolated from free polysomes and microsomes on in vitro protein synthesis in plasmacytoma cells

Autor: Abraham, K. A., Pryme, I. F., Eikhom, T. S.
Zdroj: Molecular Biology Reports; September 1974, Vol. 1 Issue: 7 p371-377, 7p
Abstrakt: A simple procedure for measuring chain initiation in complete in vitro systems is described. The capacity of free and membrane-bound polysomes prepared by a detergent technique or by nitrogen cavitation to incorporate radioactive amino acids into proteins was compared with the capacity of these polysomes to initiate polypeptide chains.
Databáze: Supplemental Index