| Autor: |
Smith, Robert J., Rohloff, Norman A., Sam, Laurel M., Justen, James M., Deibel, Martin R., Cornette, James C. |
| Zdroj: |
Inflammation; August 1989, Vol. 13 Issue: 4 p367-382, 16p |
| Abstrakt: |
Recombinant human interleukin-1a (rhIL-1a) and recombinant human interleukin 1ß (rhIL-1ß) stimulated the time- and concentration-dependent release of glycosaminoglycan (GAG) from bovine nasal cartilage expiants. Maximum GAG release occurred during six to eight days of cartilage exposure to either species of rhIL-1; and rhIL-1a was consistently more potent than rhIL-1ß. In addition to inducing cartilage matrix resorption, rhIL-1a and rhIL-1ß also inhibited the incorporation of [35SO4]sulfate into cartilage, which is a reflection of the suppression of GAG synthesis. IL-1 had no capacity to stimulate GAG relase from or inhibit GAG synthesis by dead cartilage. Cycloheximide, an inhibitor of protein synthesis, and 1, 10-phenanthroline, a metalloproteinase inhibitor, suppressed rhIL-1-stimulated cartilage matrix resorption. Polyclonal antisera to rhIL-1a and rhIL-1ß specifically neutralized the respective cytokines. |
| Databáze: |
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