Autor: |
Hou, C T, Ahlgren, J A, Brown, W, Nicholson, J J |
Zdroj: |
Journal of Industrial Microbiology and Biotechnology; February 1996, Vol. 16 Issue: 2 p129-133, 5p |
Abstrakt: |
A bacterium isolated from soil and identified asAgrobacterium sp produced a water-soluble extracellular polysaccharide capable of producing highly viscous solutions. Gas chromatographic analysis revealed a sugar composition of glucose, galactose and mannose in the molar ratio of 7.5:2.4:1, together with 3.7% (w/w) pyruvic acid. Methylation analyses showed the presence of (1?3)-, (1?4)- and (1?6)-linked glucose, (1?3)- and (1?4, 1?6)-linked galactose and a small portion of (1?3)-linked mannose residues. Succinic acid was not present. The molecular weight of the polysaccharide was estimated by light scattering to be 2×106 Da. The viscosity of solutions containing the polysaccharide remained constant from pH 3 to 11, and decreased by 50% when heated from 5 to 55°C. Maximum yield of the polysaccharide, 20 g L-1, was reached in 48 h at 30°C incubation. |
Databáze: |
Supplemental Index |
Externí odkaz: |
|