Automatic whole broth multi-fermentor sampling

Autor: Reda, K. D., Thien, M. P., Feygin, I., Marcin, C. S., Chartrain, M. M., Greasham, R. L.
Zdroj: Journal of Industrial Microbiology and Biotechnology; April 1991, Vol. 7 Issue: 3 p215-220, 6p
Abstrakt: Summary An efficient, aseptic method of obtaining whole broth fermentation samples was developed based on a piston-valve, a local sample loop, and an ability to drive the entire sample volume with sterile air through a sample line and into a remote tube. The configuration delivers 10-ml samples 10 m away with about 4 ml of broth wasted in the sampling process. An autosampler was enhanced and programmed to control acquisition into chilled tubes. The autosampler-based system represents a convenient way to provide frequent samples to profile intracellular and extracellular components for yeast and bacterial fermentations. A configuration to provide sampling from six fermentors with a multi-rack autosampler will be presented.
Databáze: Supplemental Index