Utilization of cathodically-produced hydrogen from mild steel byDesulfovibrio species with different types of hydrogenases

Autor: Czechowski, M. H., Chatelus, C., Fauque, G., Libert-Coquempot, M. F., Lespinat, P. A., Berlier, Y., LeGall, J.
Zdroj: Journal of Industrial Microbiology and Biotechnology; December 1990, Vol. 6 Issue: 4 p227-233, 7p
Abstrakt: Summary Desulfovibrio (D.) vulgaris Hildenborough with a highly active Fe-containing periplasmic hydrogenase,D. salexigens British Guiana with a Fe-Ni-Se periplasmic hydrogenase, andD. multispirans with a Fe-Ni cytoplasmic hydrogenase utilized cathodically-produced hydrogen from mild steel as the only energy source for activity and growth. Changes on the mild steel surface occurred during growth of these bacteria. The concentration of iron sulfide, a corrosion product of mild steel, increased over time, andDesulfovibrio species had an active hydrogenase when they were grown in lactate/sulfate media. This hydrogenase may be any of the three types found in the genus,Desulfovibrio. The concentration of iron in the media affected the production and activity of the Fe-hydrogenase fromD. vulgaris Hildenborough. With an iron-limited medium, the specific activity and the total amount of the periplasmic hydrogenase was less than found with a non-iron limited media.
Databáze: Supplemental Index