| Autor: |
Monaghan, Richard L., Arcuri, Edward, Baker, Edward E., Buckland, Barry C., Greasham, Randolph L., Houck, David R., Ihnen, Ernel D., Inamine, Edward S., King, Joseph J., Lesniak, Ellen, Masurekar, Prakash S., Schulman, Cheryl A., Singleton, Bert, Goetz, Michael A. |
| Zdroj: |
Journal of Industrial Microbiology and Biotechnology; March 1989, Vol. 4 Issue: 2 p97-104, 8p |
| Abstrakt: |
Summary The natural product asperlicin is the first nonpeptide antagonist of cholecystokinin isolated from a microbial source. At discovery, production of asperlicin by the original soil isolate ofAspergillus alliaceus was between 15 and 30 mg/l. Selection of natural variants ofA. alliaceus, use of Plackett & Burman and Simplex experimental designs; formulation of synthetic media; amino acid supplementation of production media; analysis of complex nitrogen sources for their amino acid content; evaluation of promising media in fermentors; substitution of glycerol for glucose as a carbon source and rational mutant selection all contributed to titer increases to >900 mg/l. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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