| Autor: |
Graham, Michael W., Doherty, Judith P., Woodcock, David M. |
| Zdroj: |
Plant Molecular Biology Reporter; February 1990, Vol. 8 Issue: 1 p18-27, 10p |
| Abstrakt: |
It has recently become apparent that many strains ofE. colicontain nucleases encoded by themcrAandmcrBloci that, recognize the modified base 5-methylcytosine in DNA. Plant DNAs have particularly high levels of this modification and the activity of these 5-methylcytosine-specific nucleases is particularly relevant to cloning plant genomic DNAs. We show here that for preparing libraries in a λ replacement vector, the use of suitablemcrhosts andmcrpackaging mixes can increase the efficiency of cloning of plant genomic DNAs by at least two orders of magnitude. We also provide evidence that the activity of themcrnucleases is probably a significant source of bias in the representation of sequences in plant genomic libraries. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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