| Autor: |
Barker, David G., Bianchi, Sylvie, Blondon, François, Dattée, Yvette, Duc, Gérard, Essad, Sadi, Flament, Pascal, Gallusci, Philippe, Génier, Gérard, Guy, Pierre, Muel, Xavier, Tourneur, Jacques, Dénarié, Jean, Huguet, Thierry |
| Zdroj: |
Plant Molecular Biology Reporter; February 1990, Vol. 8 Issue: 1 p40-49, 10p |
| Abstrakt: |
Medicago truncatulahas all the characteristics required for a concerted analysis of nitrogen-fixing symbiosis withRhizobiumusing the tools of molecular biology, cellular biology and genetics.M. truncatulais a diploid and autogamous plant has a relatively small genome, and preliminary molecular analysis suggests that allelic heterozygosity is minimal compared with the cross-fertilising tetraploid alfalfa (Medicago sativa). TheM. truncatulacultivar Jemalong is nodulated by theRhizobium melilotistrain 2011, which has already served to define many of the bacterial genes involved in symbiosis with alfalfa. A genotype of Jemalong has been identified which can be regenerated after transformation byAgrobacterium, thus allowing the analysis ofin-vitro-modified genes in an homologous transgenic system. Finally, by virtue of the diploid, self-fertilising and genetically homogeneous character ofM. truncatula, it should be relatively straightforward to screen for recessive mutations in symbiotic genes, to carry out genetic analysis, and to construct an RFLP map for this plant. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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