| Autor: |
Tokiwa, T., Lipsky, M. M., Smoot, D. T., Lechner, J. F. |
| Zdroj: |
Methods in Cell Science; March 1994, Vol. 16 Issue: 1 p57-60, 4p |
| Abstrakt: |
Summary A method is described for establishing continuous cell lines of liver epithelial cells by transformation of cultured hepatocytes isolated by collagenase/dispase of adult human liver tissue. Between 2 to 5×105 hepatocytes are inoculated into a 60 mm culture dish. The cells are incubated in a serum-free medium. Once the cells begin to divide, they are transformed by transfection with a plasmid containing SV40 early region genes. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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