Simultaneous preparation of four cell type enriched glial cultures from rat cerebral cortex

Autor: da Cunha, Anna, Vitković, Ljubiša
Zdroj: Methods in Cell Science; March 1991, Vol. 13 Issue: 1 p31-38, 8p
Abstrakt: Summary: We have developed a procedure for the stimultaneous isolation of 95 to 98% homogeneous populations of four glial cell types from a heterogeneous cell suspension derived from one pool of neonatal rat cerebral cortices. The method is based on four known differences between microglia, astrocyte types 1 and 2, and oligodendrocytes: a) timing of differentiation and preservation of this schedule in dissociated cell cultures; b) adhesion strength; c) cell size; and d) nutritional requirements. The advantages of this procedure over current methods include: a) cultures remain pure approximately 2 wk longer; b) cultures of type 2 astrocytes are approximately 25% more pure; c) direct comparisons of these cell types are made possible during development and/or in maturity under a variety of heritable and other perturbations; d) biochemical analyses of the interactive and individual properties of glial cells are facilitated; and e) animal lives are conserved. The method may prove particularly valuable in human brain research where supply of tissue is both sporadic and limited.
Databáze: Supplemental Index