A serum-free method for culturing normal human bronchial epithelial cells at clonal density

Autor: Lechner, John F., LaVeck, Moira A.
Zdroj: Methods in Cell Science; June 1985, Vol. 9 Issue: 2 p43-48, 6p
Abstrakt: Summary A technique for the isolation and culture of normal human bronchial epithelial (NHBE) cells is described. This procedure involves first explanting fragments of large airway tissue to initiate fibroblastic-cell-free outgrowths of epithelial cells and subsequently using a serum-free medium (LHC-9) to propagate the NHBE cells at clonal density.
Databáze: Supplemental Index