| Autor: |
Bolton, W. E., Terrell, S. P., Andrews, K. L., Boyd, A. E. |
| Zdroj: |
Methods in Cell Science; March 1982, Vol. 7 Issue: 1 p39-42, 4p |
| Abstrakt: |
Summary We describe a technique using normal and diabetic (dbdb) mice to establish primary pancreatic cultures that spread and assume a characteristic epithelial morphology. These cultures contain 4 to 7% beta cells, secrete insulin in response to stimuli for 10 to 14 d, contain few fibroblasts, and have a cell viability that is greater than 95%. The cells attach firmly to glass cover slips and are ideal for the study of insulin secretory granules or contractile proteins using indirect immunofluorescence. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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