Preparation of a proteolytic enzyme mixture containing trypsin, elastase, and chymotrypsin for use in tissue disaggregation

Autor: McCarl, Richard L., Kunze, Elaine L., Speicher, David W.
Zdroj: Methods in Cell Science; June 1980, Vol. 6 Issue: 2 p47-49, 3p
Abstrakt: Summary We describe a purification method for tissue culture-grade trypsin that yields an enzyme mixture with reproducible activities of trypsin, elastase, and chymotrypsin and eliminates amylase and lipase.
Databáze: Supplemental Index