| Autor: |
Tokashiki, Michiyuki, Arai, Takami |
| Zdroj: |
Cytotechnology; February 1989, Vol. 2 Issue: 1 p5-8, 4p |
| Abstrakt: |
Mouse-human hybridoma X87X cells were cultivated using a novel perfusion culture apparatus provided with three-settling zones to separate the cells from the culture medium by gravitational settling. The maximum viable cell density in a serum-free culture medium attained 3.0×107 cells/ml, when the specific perfusion rate was set to 2.3 vol day-1, and monoclonal antibody was continuously produced. These results were almost the same as those in the perfusion culture vessel with one settling zone and revealed that the process with a plurality of settling zones is a promising one for scale-up of a gravitation type of perfusion culture vessel. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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