| Autor: |
Junker, B., Wu, F., Wang, S., Waterbury, J., Hunt, G., Hennessey, J., Aunins, J., Lewis, J., Silberklang, M., Buckland, B. |
| Zdroj: |
Cytotechnology; February 1992, Vol. 9 Issue: 1-3 p173-187, 15p |
| Abstrakt: |
Abstract: Microcarrier culture was investigated for the propagation of attenuated hepatitis A vaccine in the anchorage-dependent human fibroblast cell line, MRC-5. Cells were cultivated at 37C for one to two weeks, while virus accumulation was performed at 32C over 21 to 28 days. The major development focus for the microcarrier process was the difference between the cell and virus growth phases. Virus antigen yields, growth kinetics, and cell layer/bead morphology were each examined and compared for both the microcarrier and stationary T-flask cultures. Overall, cell densities of 4–5106 cells/ml at 5–10 g/l beads were readily attained and could be maintained in the absence of infection at either 37C or 32C. Upon virus inoculation, however, substantial cell density decreases were observed as well as 2.5 to 10-fold lower per cell and per unit surface area antigen yields as compared to stationary cultures. The advantages as well as the problems presented by the microcarrier approach will be discussed. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
Full text from SpringerLink