| Autor: |
Nayve, Fidel Rey P., Motoki, Masamichi, Matsumura, Masatoshi, Kataoka, Hiroshi |
| Zdroj: |
Cytotechnology; June 1991, Vol. 6 Issue: 2 p121-130, 10p |
| Abstrakt: |
Serum-free perfusion cultures of hybridoma TO-405 cells were carried out in spinner flasks coupled with zeolite A-3 packed beads. Ammonia was selectively removed from the culture broth by passing cell free permeate from ceramic cross flow filtration, through the zeolite packed bed. Ammonia concentration in the culture broth was effectively maintained between 1 to 4 mmol/l which was below the inhibitory concentration for cell growth. Maximum cell density levels of 107 cells/ml as well as improved percentage cell viability higher than in serum-supplemented cultures were feasible in this system. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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