| Autor: |
Barnet, K., Vacek, A., Cech, K., Pekárek, J. |
| Zdroj: |
Biotherapy; March 1996, Vol. 9 Issue: 1-3 p171-174, 4p |
| Abstrakt: |
Abstract: Dialysable leucocyte extract (DLE) prepared from buffy coats of human blood, potentiates the effect of Colony-stimulating factor (CSF) on the growth of granulocyte-macrophage colony forming cell (GM-CFC) colonies in vitro. This relative increase of the number of colonies is apparent when diluted CSF (present in lung conditioning medium) as a control, and DLE, in a wide range of concentrations are added to the culture of mouse bone marrow cells. Fractionation of DLE on Amicon membranes revealed that the activity resides in molecules of 0-5kD. Molecules 5-10kD have no potentiating effect. DLE and its fractions (0-5kD, 0-1kD), except fractions 0-500 D and 5-10kD, when added undiluted i.e. at the initial concentration, exerted a suppressive effect: colonies are not formed despite the presence of CSF. In a pilot experiment, it was shown that DLE is able to stimulate colony-forming activity of earlier progenitors of erythroid cells (BFUe), under the influence of erythropoietin. |
| Databáze: |
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