| Autor: |
Kotoyori, Jun, Kozawa, Osamu, Tokuda, Haruhiko, Miwa, Masaichi, Oiso, Yutaka |
| Zdroj: |
Journal of Bone and Mineral Metabolism; September 1992, Vol. 10 Issue: 2 p1-5, 5p |
| Abstrakt: |
Summary: The effects of cAMP on insulin-like growth factor-I (IGF-I) secretion and45Ca-accumulation into extracellular matrix were investigated in cloned osteoblast-like MC3T3-E1 cells cultured for 7 weeks, which was considered to be long enough to develop calcification. Dibutyryl cAMP stimulated IGF-I secretion dose dependently in the range between 0.1 mM and 3 mM. Dibutyryl cAMP also stimulated accumulation of45Ca into sodium dodecyl sulfate-insoluble, EDTA-extractable materials in cultures of these cells dose dependently between 0.1 mM and 3 mM. The patterns of both stimulations were similar. The dibutyryl cAMP-stimulated IGF-I secretion preceded the45Ca-accumulation. Antibodies to IGF-I suppressed the dibutyryl cAMP-stimulated45Ca-accumulation almost to the control level. Taking account of our previous report that exogenous IGF-I stimulates45Ca-accumulation in cultures of these cells, the present results suggest that cAMP stimulates45Ca-accumulation via synthesis of IGF-I in osteoblast-like cells. |
| Databáze: |
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