Enzymatic synthesis and characterization of N5-(carboxymethyl)-L-ornithine and N6-(carboxymethyl)-L-lysine

Autor: Miller, S. P. F., Donkersloot, J. A., Thompson, J.
Zdroj: Amino Acids; June 1994, Vol. 6 Issue: 2 p189-198, 10p
Abstrakt: This report describes the enzyme-catalyzed synthesis, characterization, and chromatographic separation of N6-(carboxymethyl)-L-lysine and N5-(carboxymethyl)-L-ornithine. The two N?-(carboxyalkyl)amino acids are formed via a reductive condensation between glyoxylate and thee- ord-amino groups of lysine and ornithine, respectively. Both reactions are catalyzed by the NADPH-dependent enzyme, N5-(carboxyethyl)ornithine synthase [EC 1.5.1.24], found in some strains of the lactic acid bacteriumLactococcus lactis subsp.lactis.
Databáze: Supplemental Index
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