| Abstrakt: |
Five ent-kaurane diterpenoids, 6β,7β,14β-trihydroxy-1α,19-diacetoxy-7α,20-epoxy- ent-kaur-16-en-15-one ( 1), 1α,6β,7β-trihydroxy-11α,19-diacetoxy-7α,20-epoxy- ent-kaur-16-en-15-one ( 2), 6-hydroxy-1α,19-diacetoxy-6,7-seco- ent-kaur-16-en-15-one-7,20-olide ( 3), 19-hydroxy-1α,6-diacetoxy-6,7-seco- ent-kaur-16-en-15-one-7,20-olide ( 4), and 6-aldehyde-1α,19-diacetoxy-6,7-seco- ent-kaur-16-en-15-one-7,20-olide ( 5), along with 10 known ent-kaurane diterpenoids, pseurata C ( 6), longikaurin C ( 7), effusanin C ( 8), longikaurin B ( 9), longikaurin D ( 10), effusanin D ( 11), excisanin B ( 12), lasiokaurin ( 13), megathyrin A ( 14), and loxothyrin A ( 15), were isolated from the aerial parts of Isodon japonicus. Their structures were determined on the basis of spectroscopic (1D-, 2D-NMR and MS) and chemical evidence. The isolates were evaluated for their inhibitory effects on LPS-induced production of nitric oxide in murine macrophage RAW264.7 cells. |
