Autor: |
Pehu, T. M., Mäki-Valkama, T. K., Valkonen, J. P. T., Koivu, K. T., Lehto, K. M., Pehu, E. P. |
Zdroj: |
American Journal of Potato Research; September 1995, Vol. 72 Issue: 9 p523-532, 10p |
Abstrakt: |
The cloned P1 sequence of PVYOwas transferred in sense orientation into the potato cultivar Pito usingAgrobacteriummediatedtransformation. Sixteen of the putatively transformed plants (NPTII positive) were assayed for PVYOresistance. No PVYOwas detected in four plants, representing two lines, 21 days after two sap-inoculations and 35 days after graft-inoculation, and the plants remained symptomless, whereas other tested plants showed mosaic symptoms and had high PVY titers similar to those of the control plants. No line was resistant to PVYNand potato viruses A and X. Southern analysis confirmed the presence of the transgene(s) in the two PVYO-resistant and one susceptible line examined, but no signal was detected in nontransformed Pito. These results suggest a high level of protection against PVYOin potato transformed with P1 sequence of PVYO. |
Databáze: |
Supplemental Index |
Externí odkaz: |
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