| Autor: |
Langille, Alan R., Prouty, K. L., Halteman, W. A. |
| Zdroj: |
American Journal of Potato Research; October 1993, Vol. 70 Issue: 10 p735-741, 7p |
| Abstrakt: |
In an attempt to improve nutritional qualities of the potato by exploiting variation associated with protoplast regeneration, Russet Burbank leaf protoplasts were cultured in cell layer (CL) medium containing the amino acid analog, 5-methyltryptophan (5-MT) at concentrations from 0 to 286 μM. At three day intervals, numbers of viable cells and dividing colonies were recorded to determine effects of 5-MT concentration on cell viability during the 14 day incubation period. Repeated count data were used in a computer program to construct survival profiles. Decline in cell populations was generally slight during the first 5 days in culture. Between day 5 and 11, survival rate dropped dramatically, averaging 10–12% per day regardless of concentration. As surviving cells began to divide, a leveling trend in the curves was noted between days 11 and 14. Reduced plating efficiency was only associated with the 103 and 286 μM treatments. No plants were regenerated from colonies exposed to 5-MT concentrations in CL greater than 34.4 μM nor were differences in free tryptophan found between tubers of protoclones and Russet Burbank. |
| Databáze: |
Supplemental Index |
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