Effect ofS-adenosyl-L-methionine and its analogues on site-specific binding of DNA-(adenine-N6)-methyltransferase of T4 phage with the oligonucleotide substrate

Autor: Evdokimov, A. A., Zinoviev, V. V., Malygin, E. G.
Zdroj: Russian Journal of Bioorganic Chemistry; November 2000, Vol. 26 Issue: 10 p716-719, 4p
Abstrakt: Using fluorescence of 2-aminopurine-substituted oligonucleotide duplexes, “flipping” of the target base in the process of interaction of T4 DNA-(adenine-N6)-methyltransferase (EC 2.1.1.72) with the substrate double-stranded DNA was revealed. It was shown thatS-adenosyl-L-methionine, the methyl group donor, induces the reorientation of the enzyme relative to the unsymmetrically modified recognition site.
Databáze: Supplemental Index
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