| Autor: |
Hamilton, T E, Lawrence, P J |
| Zdroj: |
Antimicrobial Agents and Chemotherapy; July 1975, Vol. 8 Issue: 1 p38-44, 7p |
| Abstrakt: |
Triton X-100 treatment or freeze-thawing damages the membranes of sporulating or vegetative cells as seen by protein leakage from cells. A 40% increase in the specific [(14)C]benzylpenicillin-binding capacity of detergent-treated or frozen sporulating cells was observed. Neither freezing nor Triton X-100 treatment of vegetative cells produced a detectable effect on their [(14)C]benzylpenicillin-binding capacity. These data indicate the presence of penicillin-binding sites in intact sporulating bacilli not accessible to penicillin in routine binding assays. The chemical specificity of [(14)C]benzylpenicillin binding to detergent-treated sporulating cells is similar to that observed with untreated vegetative or sporulating cells. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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