| Autor: |
Agronenko, V. A., Belkin, A. L., Tibilova, N. N., Pendrak, K. A., Povzhitkova, M. S., Petrenko, S. V., Tsisarenko, T. A., Markova, N. A., Platonova, O. V., Ermolchuk, O. N. |
| Zdroj: |
Artificial Cells, Blood Substitutes, and Biotechnology; 1987, Vol. 15 Issue: 1 p237-248, 12p |
| Abstrakt: |
Incubation of long-preserved donor blood erythrocytes (over 21 days) with rejuvenating solution Erythropifaden resulted in an increased ATP concentration to 2.03±0.34 mmol/l; increased 2,3-DPG to 3.89±0.51 mmol/l, and the number of erythrocyte discoid forms was raised to 30%. Further blood perfusion through SKN-D hemosorbent allowed to reduce the following adenine level to zero; riboxine by 90; citrate and lactate by 50%. The plasma K+ was decreased from 29.8±1.25 to 13.0±0.87 mmol/l, while blood pH rose from 6.675±0.018 to 7.310±0.028. At the end of the perfusion, the ATP level was seen to further increase to 2.53±0.28 mmol/l, 2 and 3-DPG to 4.99±0.7 mmol/l. The indices of osmotic resistance and deformability of erythrocytes were normalized. The discocyte number reached 40%. The amount of red blood cell irreversible forms declined from 26 to 13% without any increment plasma free hemoglobin. The data obtained confirm the high efficacy of the combination of the rejuvenating and hemosorption procedures when applied to stored long-preserved blood. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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