| Autor: |
Rumijowska, A., Lisowska, K., Ziótkowski, A., Sedlaczek, L. |
| Zdroj: |
World Journal of Microbiology and Biotechnology; January 1997, Vol. 13 Issue: 1 p89-95, 7p |
| Abstrakt: |
An enhancement of Β-sitosterol transformation to androstendione byMycobacterium vaccaeobserved in medium containing egg-yolk lecithin, was associated with the incorporation of a considerable amount of lecithin into the cell envelope lipids. By GC/MS measurements, fatty acids ranging from 14 to 22 carbon atoms were identified in the lipids removed from the cells by organic solvents. Octadecenoic (18:1), 2-methyl-octadecenoic (2-Me 18:1), and hexadecanoic (16:0) acids were the major components of the lipid preparation obtained from both the control cells, and the cells grown in lecithin-containing medium. However, in the fatty acid pattern of the latter a distinct increase in the C18:1component, concomitant with the decrease in the 2-Me 18:1 fatty acid was demonstrated. The C16fatty acid fraction also showed a higher content of methyl-branched components in the control cell preparation. The enrichment in unsaturated fatty acids increases fluidity of lipids, whereas the decrease in methyl-branched fatty acids may affect the conformation of the surface lipidic components, which may result in enhanced sterol penetration through the cell wall barrier in the presence of lecithin. |
| Databáze: |
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