Gram Reaction in Crushed Yeasts

Autor: Kemp, Hardy
Zdroj: Biotechnic & Histochemistry; 1931, Vol. 6 Issue: 2 p53-56, 4p
Abstrakt: Thick smears of yeasts are dried, crushed between slides or cover-slips, stained with anilin crystal violet 11/2 minute, Lugol's iodine solution 2 minutes, decolorized in undiluted anilin oil, and counterstained with 1% aqueous safranin. Photomicrographs are given of crushed yeasts, thus stained, which show a Gram-positive outer layer and a Gram-negative inner substance.
Databáze: Supplemental Index