Synthesis and fluorescent properties of 5-(1-pyrenylethynyl)-2′-deoxyuridine-containing oligodeoxynucleotides

Autor: Malakhov, A. D., Malakhova, E. V., Kuznitsova, S. V., Grechishnikova, I. V., Prokhorenko, I. A., Skorobogatyi, M. V., Korshun, V. A., Berlin, Yu. A.
Zdroj: Russian Journal of Bioorganic Chemistry; January 2000, Vol. 26 Issue: 1 p34-44, 11p
Abstrakt: Novel reagents for the fluorescent labeling of oligo- and polynucleotides have been prepared: 5-(1-pyrenylethynyl)-2′-deoxyuridine 3′-phosphoramidite and a solid support carrying this nucleoside. Oligo-nucleotides containing one or several modified units have been synthesized, and the fluorescence of these probes has been shown to change upon hybridization with the complementary sequence.
Databáze: Supplemental Index