Short-step chemical synthesis of DNA by use of MMTrS group for protection of 5 hydroxyl group

Autor: Shiraishi, Miyuki, Utagawa, Eri, Ohkubo, Akihiro, Sekine, Mitsuo, Seio, Kohji
Zdroj: Nucleic Acids Symposium Series; November 2007, Vol. 51 Issue: 1 p129-130, 2p
Abstrakt: 4-methoxytrithylthio (MMTrS) group was applied for the appropriately protected four canonical nucleosides. We prepared the phosphoroamidite units by use of these nucleosides and developed the synthesis of oligodeoxynucleotides without any acidic treatment. Moreover, the new DNA synthesis protocol was applied to an automated DNA synthesizer for the synthesis of longer oligodeoxynucleotides.
Databáze: Supplemental Index