| Abstrakt: |
Recently, we described the antisense activity of 2’,4’-BNA oligonucleotides in living cells.1 Here, we examine the antisense effect of 2’,4’-BNA, 2’,4’-COC and 3’-amino-2’,4’-BNA oligonucleotides targeting the bcl-xLgene. The results showed that while S-oligo had a slightly inhibiting effect on bcl-xLexpression and natural DNA had no effect, an antisense oligonucleotide (AON) containing six 2’,4’-COC nucleotides reduced the bcl-xLmRNA level to 64% of the untreated level. Interestingly, several types of AON—those containing nine 2’,4’-BNACOC nucleotides, 2’,4’-BNA and 3’-amino-2’,4’-BNA— completely inhibited expression of the target gene. These data show that, compared with the corresponding natural DNA and S-oligo, BNA-based AONs are efficient inhibitors of bcl-xLexpression. |
